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PRESCRIPTION FOR LONGEVITY: FADS AND REALITY INTRODUCTION

At a workshop held on November 21-23, 1997 and co-sponsored by the International Longevity Center and Canyon Ranch in Tucson, AZ, 14 leading gerontologists and other health scientists met to discuss what we currently know about how diet, dietary supplements, exercise and other interventions contribute to better health and increased longevity in older people.
This group particularly addressed the question: Which of the much hyped dietary supplements are truly effective AND safe? The purpose of these workshop discussions was to write a rational prescription with respect to effectiveness and safety, and to develop a research agenda for future work in order to provide facts to replace fads.

Exercise

Americans on the average do not get enough exercise, according to our current understanding of optimal levels for longevity. Although it is not known whether there is an exercise threshold below which little benefit would be provided, a minimum increase of at least 1000 kcal per week above sedentary baseline levels is recommended. A level of 2000-3000 kcal is preferable, but may not be possible for the very frail old. Both endurance and resistance training are recommended, but the optimal amount of exercise may vary among individuals based on illness, age, physical limitations, etc. Heavy exercise should be avoided in the early morning for people at risk for myocardial infarction, and in the late evening, because it may interfere with the ability to fall asleep. The optimal time is usually the late afternoon for most people.

Diet

The following practices are recommended: Calcium should be supplemented, if necessary, to ensure a daily intake of 1200 mg for men and 1500 mg for women. Include at least 2 to 4 helpings of fruits and 3 to 5 helpings of vegetables per day, Include one multi-vitamin tablet per day to ensure adequate intake of vitamins B6, B12, C, D, E, and folic acid, but for the elderly, containing no or low amounts of iron,* Reduce fat to 30% or less of total caloric intake. * A diet rich in fruits and vegetables is preferred over dietary supplements, but the diets of many older Americans are deficient in one or more of these micronutrients, and may not supply the optimal amount of some micronutrients, e.g. vitamin E. Hormone replacement therapy Estrogen replacement therapy is justified for many post-menopausal women (after consultation with a physician, and in the absence of contra-indications). No other hormone intervention is recommended at this time.

Sleep

Older people with sleeping problems should be evaluated and treated accordingly, including the possible use of melatonin or light exposure to reset the sleep-wake cycle. Other
  • Regular sun exposure is recommended to maintain adequate vitamin D levels and/or the sleep-wake cycle, although excessive exposure should be avoided to reduce risk of skin cancer and/or melanoma.
  • Obesity at any age should be avoided, but it is also important to prevent excessive weight gain with increasing age.
  • Include one children’s aspirin (81 mg) per day to reduce risk of myocardial infarction and stroke.
  • Stress reduction/relaxation, e.g. exercise, Yoga, Tai Chi, etc.
  • Genetic interventions, while promising in animal models, appear to be well into the future, if at all, for humans.

    FUTURE RESEARCH

    Additional research is needed to:
  • Identify healthy weight ranges for individuals over 65 years of age
  • Improve our knowledge about optimal intake of both macronutrients and micro-nutrients for better health for individuals over 65 years of age, and for longevity,
  • Obtain reliable biomarkers of human aging to provide information about biological rather than chronological age; not all systems can be expected to age at the same physiological rate
  • Obtain better information on the influence of various antioxidants, both alone and in combination, on overall health and longevity
  • Obtain more information on regulation of sleep in older individuals in order to develop individualized intervention strategies,
  • Obtain better information about glucose intolerance and insulin resistance, and how these can be modulated by dietary interventions
  • Define subgroups needing specific hormone replacement therapies, and develop effective and safe hormone-based interventions
  • Develop strategies to educate the public about healthy dietary and exercise habits, including the importance of adequate sleep, and encourage changing behaviors. PROCEEDINGS OF THE WORKSHOP Physical Activity Physical activity is a complex phenomenon, that to be understood must be characterized by task, intensity, duration, and frequency. On the average, most Americans of all ages do not get sufficient physical activity. Coupled with this observation is the extensive support for the effectiveness of physical activity in the reduction in structural and functional declines that occur with aging. The structural decline is most evident in muscle atrophy which results in a loss of 30% to 40% of the muscle mass between 30 and 70 years of age. The functional declines in endurance and strength are even greater. At any given level of conditioning, a threshold exists for the intensity, duration, and frequency of each task below which no structural or functional benefit is provided. Despite the complexity of this analysis, for most adults, a minimum increase of at least 1000 kcal per week above sedentary baseline levels is recommended, which is equivalent to walking four miles a day, five times a week. A level of 2000-3000 kcal above baseline is preferable, which translates to jogging rather than walking the same distance and frequency. Both endurance conditioning for the cardiovascular and respiratory systems, and resistance conditioning for strength, are required to achieve or maintain total body fitness. The optimal type of task, intensity, duration, and frequency of physical activity will vary among individuals based on illness, age, physical limitations, and current state of conditioning. Heavy exercise should be avoided in the early morning for people at risk for having a myocardial infarction, and in the late evening, because it may interfere with the ability to fall asleep. For most people, the optimal time to exercise vigorously is late afternoon.

    Exercise

    With increasing age, the muscles atrophy and become weaker and more fatiguable. Superficially, these changes that occur with age appear similar to the changes that occur at any age when a decrease in physical activity occurs. The difference is that the loss in muscle mass and the decreases in strength and endurance associated with inactivity are totally reversible with subsequent conditioning, whereas the changes associated with aging are observed in both highly conditioned and sedentary people. Importantly, the rate of change is much more rapid when aging is coupled with decreasing physical activity. Under these circumstances, the cumulative losses in muscle mass and function may lead to a loss in mobility, an increased susceptibility to falls and ultimately severe disabilities for older persons. Although under certain conditions growth hormone may increase muscle mass of elderly males, the increase depends on continuing the treatment, which is both expensive and may be accompanied by adverse health effects. Regular physical activity and adequate nutrition provide a much more effective intervention strategy for the elderly to maintain not only an adequate muscle mass, but also muscles that function properly. Skeletal muscles are capable of performing three types of contractions: miometric contractions when the muscle shortens, isometric when the muscle remains at the same length, and pliometric when the muscle is stretched. For normal function of muscles to be maintained in the elderly, the muscles of the upper and lower limbs and of the trunk must perform each type of contraction frequently and regularly. If muscles do not perform each type of contraction frequently and regularly the muscle fibers that make up the muscle undergo changes that make them more susceptible to injury, and subsequent physical activity is even more difficult. The fibers in the muscles of old people are more easily injured by their own contractions than those in young or adult people. Consequently, regular strength conditioning of muscles is even more important for the elderly than for younger people. The only way to acquire and maintain strength conditioned muscles is to exercise with a diversity of tasks of lifting and lowering activities with light weights that involve the muscles of the arms, legs and trunk. Each individual task for a specific muscle group should be performed 10 times at about 80% of a maximum effort and then repeated 3 times with rests in between. The second requirement of total body fitness for the elderly is to maintain a reasonable level of total body endurance. Total body endurance which loads the cardiovascular and respiratory systems is achieved most easily and effectively by a total body activity such as walking, jogging, crosscountry skiing, skating, or cycling. For a 70 kg person, walking is about 200 kcal/hour above baseline, and slow jogging is about 500 kcal/hour above baseline. These activities involve the moving of the body mass; consequently, the caloric cost will go up or down proportionately with greater or lesser body masses.

    Oxidative Damage and Age-related Disease

    All living cells are exposed to oxidative stress in the form of oxygen radicals. In most eukaryotic cells this occurs primarily during the metabolism of oxygen by the electron transport system in the mitochondria. Because of this continuous exposure to oxygen radicals, living organisms have developed robust antioxidant defenses and systems to repair damaged proteins, DNA and unsaturated lipids. Failure to adequately deal with these oxygen radicals is a risk factor for a variety of age-related diseases such as cancer, neurodegenerative diseases, atherosclerosis, and cataract. The antioxidant defense systems include both non-specific dietary antioxidants such as vitamins C (ascorbic acid) and E, as well as specific enzymes for destroying oxygen entities such as superoxide anion and hydrogen peroxide. Thus, dietary antioxidants provide one possible opportunity for intervention. Ascorbic acid is particularly protective in smokers, and has been shown to reduce DNA damage in sperm. Very recent data suggest that ascorbic acid accumulates in neutrophils during infection, possibly to protect the neutrophils against the very oxygen radicals they generate to kill bacteria. Another opportunity, as yet largely unexploited, would be to attenuate the rate of production of oxygen radicals by mitochondria. One possible approach is to preserve as much as possible the structural integrity of mitochondria during aging of the cell. Early results in this area of research suggest that maintaining: 1) cardiolipin levels in mitochondrial membranes, and 2) acetyl carnitine synthesis in mitochondria by carnitine supplementation, may both be effective. Lipoic acid and radical scavengers such as phenyl butyl nitrone (PBN) have also been shown to reduce oxygen radical generation by mitochondria. Diet Dietary deficiencies are a well-known risk factor for many diseases, including age-related diseases such as cancer, cardiovascular disease, and osteoporosis. Epidemiological data on dietary intakes indicate that in persons whose diet is rich in fruits and vegetables, the risk of a variety of cancers is lowered by one-half. Epidemiological health data also indicate that overweight becomes an increasing problem in both men and women 40 years of age and greater, but especially in women. The percentage of individuals who are overweight reaches as high as 60% in black women in their 50s and 60s. Hypertension also increases steadily across the decades, with up to 60% of men and 80% of women being mildly to moderately hypertensive by their 6th decade. The workshop participants discussed what is currently known about the role of both micronutrients and macronutrients in human health. Particular emphasis was placed on micronutrients which may be deficient in the diet of older persons. There was consensus that dietary intakes of the following micronutrients may be inadequate in at least 10% of individuals over 70 years of age:
  • Vitamin B12 particularly important in synthesis of methionine
  • Vitamin B6 required for many reactions involving amino acids
  • Folic acid required for metabolism of groups containing one carbon atom
  • Vitamin D required for calcium absorption and bone formation
  • Calcium required for bone formation Current estimates of optimal daily intake by humans are listed in Table 1. Although micronutrients may not fall below the RDA, research has indicated that supplementation can reduce the risk of agerelated disease. For example, high levels of folate and vitamin B6 have recently been shown to reduce the risk of heart disease in women. Vitamin C has been particularly implicated in the reduction of smoking-induced oxidative damage, whereas vitamin E supplementation has been shown to reduce the risk of cancer and cardiovascular disease. The antioxidant b-carotene may actually increase the risk of cancer, at least in smokers, so its value as a dietary supplement is currently controversial. Whereas all of the above can be taken as dietary supplements, most experts agree that including generous amounts of fruits and vegetables in the diet is preferred over dietary supplementation. Current recommendations are to include at least 5 servings of fruits and vegetables per day in the diet. A less desirable alternative is to recommend a multi-vitamin pill to the public in general.

    TABLE 1

    SUGGESTED DAILY INTAKES OF MICRONUTRIENTS NUTRIENT RECOMMENDED TOTAL DAILY INTAKE
    • vitamin B6 4 mg
    • vitamin B12 10 ug
    • calcium 1200 mg for men/1500 mg for women
    • vitamin C 200 mg
    • vitamin D 400 I.U. for age < 70/600 for age > 70
    • vitamin E 200 I.U.
    • folic acid 400 ug

    TABLE 2

    COMPOSITION OF A MULTI-VITAMIN (SPECIALIZED FOR ADULTS OVER 50) AVAILABLE AT A MAJOR GROCERY CHAIN FOR LESS THAN 10c/TABLET
    NUTRITION FACTS SERVING SIZE 1 TABLET EACH TABLET CONTAINS % DAILY VALUE
  • VITAMIN A (33% AS BETA CAROTENE) 6,000 I.U. 120%
  • VITAMIN C (ASCORBIC ACID) 60 MG 100%
  • VITAMIN D 400 I.U. 100%
  • VITAMIN E 45 I.U. 150%
  • VITAMIN K 10 MCG 13%
  • THIAMIN (VITAMIN B1) 1.5 MG 100%
  • RIBOFLAVIN (VITAMIN B2) 1.7 MG 100%
  • NIACIN (NIACINAMIDE ) 20 MG 100%
  • VITAMIN B6 3 MG 150%
  • FOLATE (FOLIC ACID) 200 MCG 50%
  • VITAMIN B12 25 MCG 417%
  • BIOTIN 30 MCG 10%
  • PANTOTHENIC ACID 10 MG 100%
  • CALCIUM (CALCIUM CARBONATE) 200 MG 20%
  • IRON (FERROUS FUMARATE) 9 MG 50%
  • IODINE (POTASSIUM IODIDE) 150 MCG 100%
  • MAGNESIUM (MAGNESIUM OXIDE) 100 MG 25%
  • ZINC (ZINC OXIDE) 15 MG 100%
  • SELENIUM (SODIUM SELENATE) 20 MCG 29%
  • COPPER (CUPRIC OXIDE) 2 MG 100%
  • MANGANESE (MANGANESE SULFATE) 2.5 MG 71%
  • CHROMIUM (CHROMIUM CHLORIDE) 100 MCG 77%
  • MOLYBDENUM (SODIUM MOLYBDATE) 25 MCG 16%
  • PHOSPHORUS (CALCIUM PHOSPHATE) 48 MG 5%
    SUGGESTED USE: ADULTS, AS A DIETARY SUPPLEMENT, ONE TABLET DAILY
    There is no evidence that this would be harmful, and it is an inexpensive (5- 10c/day/person) and simple approach (see Table 2 for the composition of a multi-vitamin tablet currently available). It can be argued that the wide variations among individuals, especially among older adults, requires an individualized assessment of dietary deficiencies. The data available to date indicate that use of dietary supplements is greater in women than in men, and that both increase with increasing age in the American population.
    Other dietary supplements such as chromium ion (Cr+3), melatonin and dehydroepiandrosterone (DHEA) were also discussed. The use of Cr+3 in reducing blood sugar levels, blood pressure and lipid peroxidation in individuals with glucose intolerance and insulin resistance is based mainly on animal experiments. Recent human studies from China show that giving trivalent chromium to type 2 diabetics lessened the glucose intolerance and reduced the levels of circulating glycated hemoglobin significantly.
    Many multi-vitamin pills may not contain adequate levels of absorbable Cr+3. The participants agreed that the data in support of including melatonin as a supplement are very weak, and probably flawed. The data for DHEA are also unconvincing, and several laboratories have been unable to replicate some of the published positive results about restoring immune function in mice; DHEA also does not extend life span in mice.
    Epidemiological data on macronutrient intake were also discussed. Restriction of caloric intakes is the only known intervention which reliably extends life span and delays age-related disease in a variety of animal species. However, this intervention has yet to be adequately tested for either effectiveness or safety in humans. Although countries in which total caloric intake is high tend to have longer-lived populations, this cannot be construed to mean that caloric restriction would not work in humans. It is more likely an expression of the better overall health and better socioeconomic status of these populations. Modest reduction of total dietary fat (to no more than 30% of total calories), saturated fat (less than 10% of total calories), and cholesterol (less than 300 mg daily), consistent with the Dietary Guidelines for Americans and the Food Guide Pyramid, is important for achieving and maintaining healthy weight and reducing the risk of heart disease and certain cancers. Additional research is needed to clarify the health benefits and potential adverse effects of aggressive fat reduction beyond these targets. Additional data are also needed on the effects of modifying fat composition, i.e. saturated vs. monounsaturated and polyunsaturated fatty acids, and altering carbohydrate intake. Excessive reduction of dietary fat, with a concomitant increase in dietary carbohydrate, has been associated in some population groups with adverse effects on biomarkers of chronic disease risk, including decreased HDL-cholesterol, increased LDL-cholesterol and triglycerides, and insulin resistance. These findings may necessitate a shift in paradigm away from the central focus on dietary fat reduction, to consider the role of dietary fat and carbohydrate composition.

    Endocrine Factors

    Because hormones are produced in one part of the body and utilized at some other place, and because many hormone levels decrease with age, hormone replacement therapy is an appealing anti-aging intervention (see Table 3). The most successful example so far is estrogen replacement therapy following menopause. This therapy has proven to be not only efficacious and relatively inexpensive in lowering the risk of cardiovascular disease and osteoporosis, but also to have unanticipated consequences, such as a possible lowering of the risk of Alzheimer’s disease. A useful intervention, but one less successful over the long term includes the use of dopamine for treatment of Parkinson disease. Some success in reforming muscle mass has also been reported using growth hormone.
    Growth hormone levels dramatically decrease with age. Reducing the symptoms of Alzheimer’s disease with anti-inflammatory agents and/or regulation of the level of the neurotransmitter acetylcholine, has also been reported. A profound difficulty with these approaches is that it would be extremely difficult to mimic the cyclical changes in blood level for hormones such as growth hormone. This difficulty can lead to undesirable side effects. A conceptual complication is that just because serum concentrations of any circulating hormone decrease with age, this does not mean that restoring it to levels found in younger individuals will be beneficial. Furthermore, many of the therapies of this kind are likely to require individual assessment and surveillance, and thus be expensive and not available to the general population. Thus, only a few of these

    TABLE 3

    POTENTIAL HORMONE REPLACEMENT THERAPIES HORMONE TARGET KNOWN KNOWN EFFICACY SIDE EFFECTS
  • Estrogen Osteoporosis +++ Yes
  • Dopamine Parkinson disease ++ (short term)
  • Acetylcholine Alzheimer’s disease +
  • Growth hormone Muscle mass + Yes
  • Melatonin Sleep cycle/other ? +/?
  • DHEA Several ? Yes
  • Testosterone Several ? Yes
  • Insulin-like growth factor I Several ? Cortisol Stress ? strategies can be expected to develop into broadly applicable interventions.

    Sleep

    Well-documented sleep problems for many older adults are insomnia, sleep apnea, and changes in sleep cycles. These problems have different causes, and need to be treated differently. In the case of insomia which delays sleep until the early morning hours, taking melatonin in the late evening may advance the 24 hour biological clock sufficiently to be useful. However, melatonin is not useful for general insomnia, such as may be caused by sleep apnea. In those individuals who tend to fall asleep early in the evening, bright light in the evening may be useful for delaying the time of sleep-onset and waking. Amino acid supplements, e.g. tryptophan, do not appear to be a useful therapy, may actually contain dangerous contaminants, and are not encouraged.
    Sleep is also associated with growth hormone production, and good sleep cycling may improve growth hormone cyclicity. Thus, this issue is a significant quality of life concern in older adults.

    Genetics and Gene Therapy

    While it is obvious that differences in life span among animal species have a strong genetic basis, it has been estimated that only about 30-35% of individual differences in human longevity depend on genetic factors.
    However, with the possible exception of the gene for Werner’s syndrome, little is known about which human genes play critical roles in these differences. It can be assumed that longevity is an extremely polygenic trait, and that small differences such as single nucleotide polymorphisms (SNPs) in many genes are partially responsible for individual differences in aging.

    Until the existence and frequency of SNPs in the human genome and their impact on aging can be evaluated, an alternate approach is to try to identify and characterize candidate aging genes in genetically-pliable model systems such as yeast (S. cerevisiae), fruit flies (Drosophila), nematodes (C. elegans), and mice.
    Some progress has been made in this comparative biology endeavor. The most notable examples are the lag1 gene in yeast and the age1, daf2 and daf16 genes in nematodes. All of these genes code for proteins which play a role in overall metabolism or signal transduction pathways, suggesting there is an important link between longevity and regulation of metabolism
    The ability to elucidate how these genetic changes are translated into extended longevity in these model organisms may eventually suggest useful interventions in humans. A somewhat different, but also promising approach, has been to overexpress genes whose products either protect cells against damage or repair damage once it has occurred, e.g. antioxidant enzymes.
    Gene therapy is a much heralded, but as yet largely unfulfilled strategy for delaying, reducing or preventing age-related human pathology. Few, if any of the experimental approaches to gene therapy or clinical trials have yet led to a long term successful intervention in humans.
    The major problems appear to be selective delivery and regulated expression of the gene in the tissue of interest. Most promising are approaches to metabolize, or synthesize and secrete, circulating compounds such as hormones and cytokines. However, interventions practical for large numbers of people are clearly well in the future.